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renilla luciferase plasmid prl-null  (Promega)

 
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    Structured Review

    Promega renilla luciferase plasmid prl-null
    (A-B) IFN-β and NF-κB promoter activities are downregulated in DF-1 TLR3 KO cells but can be rescued by exogenous expression of TLR3. DF-1 and DF-1 TLR3 KO cells were co-transfected with pLucter (100 ng) and pR-null (30 ng) plasmids (A) , or with pSI-chNFκB-Luc (50 ng) plasmid (B) together with different amounts (100, 200, 400 or 800 ng) of the plasmid expressing chTLR3-His. At 8 h pt the cells were transfected with 250 ng of Poly I:C, and harvested at 24 h after plasmid transfection. A control consisting in cells co-transfected with pLucter, pR-null and the empty pCDNA3, or with the pSI-chNFκB-Luc, and the empty pCDNA3 plasmid (800 ng) was included in each assay. The samples were analyzed by the dual <t>luciferase</t> assay kit. Each determination was carried out in duplicate, and the firefly luciferase expression level of each sample was normalized by <t>Renilla</t> values. Results are expressed as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not transfected with poly I:C. (C-D) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) (C) or with the plasmid pSI-chNFκB-Luc (50 ng) (D). At 8 h pt, cells were either treated with Poly I:C (20 µg) added directly to the culture medium or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase activity quantification. (E) DF-1 and DF-1 TLR3 KO cells were transfected with a siRNA specifically silencing the expression of MDA5 protein, or with a control siRNA. At 24 h pt, the cultures were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) and 8 h later cells were either treated with poly I:C (20μg) or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase assay. (F) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng). At 8 h pt, cells were transfected with 250 ng of Poly I:C and subsequently treated with BFA (50μM) or the vehicle DMSO 1 h later. The samples were harvested at 24 h after plasmid transfection and analyzed using the dual luciferase assay kit. In panels C-F values are presented as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not treated with Poly I:C. Bars indicate means ± standard deviations based on data of duplicate samples from three independent experiments. *, ** and *** indicate p values of <0.05, <0.01 and <0.001, respectively, as determined by unpaired Student’s test. ns, not significant.
    Renilla Luciferase Plasmid Prl Null, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prl-null+plasmid/pgl3+basic/bio_rxiv__2025__07__16__665101-365-1-5
    Average 90 stars, based on 1 article reviews
    renilla luciferase plasmid prl-null - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Key role of TLR3 in type I IFN expression and apoptosis induction in IBDV-infected chicken fibroblast cells"

    Article Title: Key role of TLR3 in type I IFN expression and apoptosis induction in IBDV-infected chicken fibroblast cells

    Journal: bioRxiv

    doi: 10.1101/2025.07.16.665101

    (A-B) IFN-β and NF-κB promoter activities are downregulated in DF-1 TLR3 KO cells but can be rescued by exogenous expression of TLR3. DF-1 and DF-1 TLR3 KO cells were co-transfected with pLucter (100 ng) and pR-null (30 ng) plasmids (A) , or with pSI-chNFκB-Luc (50 ng) plasmid (B) together with different amounts (100, 200, 400 or 800 ng) of the plasmid expressing chTLR3-His. At 8 h pt the cells were transfected with 250 ng of Poly I:C, and harvested at 24 h after plasmid transfection. A control consisting in cells co-transfected with pLucter, pR-null and the empty pCDNA3, or with the pSI-chNFκB-Luc, and the empty pCDNA3 plasmid (800 ng) was included in each assay. The samples were analyzed by the dual luciferase assay kit. Each determination was carried out in duplicate, and the firefly luciferase expression level of each sample was normalized by Renilla values. Results are expressed as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not transfected with poly I:C. (C-D) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) (C) or with the plasmid pSI-chNFκB-Luc (50 ng) (D). At 8 h pt, cells were either treated with Poly I:C (20 µg) added directly to the culture medium or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase activity quantification. (E) DF-1 and DF-1 TLR3 KO cells were transfected with a siRNA specifically silencing the expression of MDA5 protein, or with a control siRNA. At 24 h pt, the cultures were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) and 8 h later cells were either treated with poly I:C (20μg) or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase assay. (F) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng). At 8 h pt, cells were transfected with 250 ng of Poly I:C and subsequently treated with BFA (50μM) or the vehicle DMSO 1 h later. The samples were harvested at 24 h after plasmid transfection and analyzed using the dual luciferase assay kit. In panels C-F values are presented as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not treated with Poly I:C. Bars indicate means ± standard deviations based on data of duplicate samples from three independent experiments. *, ** and *** indicate p values of <0.05, <0.01 and <0.001, respectively, as determined by unpaired Student’s test. ns, not significant.
    Figure Legend Snippet: (A-B) IFN-β and NF-κB promoter activities are downregulated in DF-1 TLR3 KO cells but can be rescued by exogenous expression of TLR3. DF-1 and DF-1 TLR3 KO cells were co-transfected with pLucter (100 ng) and pR-null (30 ng) plasmids (A) , or with pSI-chNFκB-Luc (50 ng) plasmid (B) together with different amounts (100, 200, 400 or 800 ng) of the plasmid expressing chTLR3-His. At 8 h pt the cells were transfected with 250 ng of Poly I:C, and harvested at 24 h after plasmid transfection. A control consisting in cells co-transfected with pLucter, pR-null and the empty pCDNA3, or with the pSI-chNFκB-Luc, and the empty pCDNA3 plasmid (800 ng) was included in each assay. The samples were analyzed by the dual luciferase assay kit. Each determination was carried out in duplicate, and the firefly luciferase expression level of each sample was normalized by Renilla values. Results are expressed as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not transfected with poly I:C. (C-D) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) (C) or with the plasmid pSI-chNFκB-Luc (50 ng) (D). At 8 h pt, cells were either treated with Poly I:C (20 µg) added directly to the culture medium or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase activity quantification. (E) DF-1 and DF-1 TLR3 KO cells were transfected with a siRNA specifically silencing the expression of MDA5 protein, or with a control siRNA. At 24 h pt, the cultures were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) and 8 h later cells were either treated with poly I:C (20μg) or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase assay. (F) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng). At 8 h pt, cells were transfected with 250 ng of Poly I:C and subsequently treated with BFA (50μM) or the vehicle DMSO 1 h later. The samples were harvested at 24 h after plasmid transfection and analyzed using the dual luciferase assay kit. In panels C-F values are presented as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not treated with Poly I:C. Bars indicate means ± standard deviations based on data of duplicate samples from three independent experiments. *, ** and *** indicate p values of <0.05, <0.01 and <0.001, respectively, as determined by unpaired Student’s test. ns, not significant.

    Techniques Used: Expressing, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay

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    Transfection:

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    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
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    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

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    Expressing:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

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    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
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    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
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    Construct:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
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    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
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    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Plasmid Preparation:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Activity Assay:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Luciferase:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Isolation:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Sequencing:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Clone Assay:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.

    Control:

    Article Title: A PB1-K577E Mutation in H9N2 Influenza Virus Increases Polymerase Activity and Pathogenicity in Mice
    Article Snippet: The pRL-null plasmid (Promega KK, Tokyo, Japan) encodes Renilla luciferase and was utilized as an internal control to normalize transfection efficiency.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells.
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity ofHALpromoter,HepG2cellswere co-transfectedwithHAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: Crosstalk between hepatitis B virus X and high‐mobility group box 1 facilitates autophagy in hepatocytes
    Article Snippet: For the luciferase assay, cells were transiently cotransfected with the pRL‐null plasmid (Promega) containing the Renilla luciferase gene, which is used for internal normalization, and a construct containing the SP1‐binding region.

    Article Title: A T-cell-specific CD154 transcriptional enhancer located just upstream of the promoter
    Article Snippet: 7 Transfection efficiency was corrected for by measuring Renilla luciferase activity (Promega) from a cotransfected pRL-null plasmid (Promega) as described, 33 and results are presented as means±s.e.m.

    Article Title: Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells
    Article Snippet: We cloned the 497 bp promoter DNA sequence (-187 to +310) of human BRCA2 gene in front of Renilla luciferase ( Rluc ) gene in pRL-Null plasmid (Promega) to obtain clones with the insert either in the forward or in the reverse orientation with respect to the luciferase gene (Fig. ).

    Article Title: Molecular characterization and stage-dependent gene expression of gonadotropin receptors in Pacific bluefin tuna, Thunnus orientalis, ovarian follicles.
    Article Snippet: To understand the physiological mechanisms by which pituitary-derived gonadotropins (Gths), folliclestimulating hormone (Fsh) and luteinizing hormone (Lh) regulate asynchronous oocyte development, we investigated the function and expression of Fsh and Lh receptors (Fshr and Lhr, respectively) in Pacific bluefin tuna (PBT, Thunnus orientalis).. As a first, we cloned the full-length cDNAs encoding PBT Fshr and Lhr.. Recombinant PBT Fsh and Lh single-chain proteins were produced in abundance using stable CHO-DG44 cell lines and were subsequently purified from the culture medium, culminating in their yields being 87.0 and 88.2%, respectively.

    Article Title: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells
    Article Snippet: To examine the effect of FOXA1, FOXA3, and CEBPA on the activity of HAL promoter, HepG2 cells were co-transfected with HAL reporter plasmid (0.2 μg) and pRL-null plasmid (0.05 μg) in combination with plasmids expressing FOXA1 (0.2 μg), FOXA3 (0.2 μg), or CEBPA (0.02 μg) using FuGENE6 reagent (Promega).

    Article Title: The NKG2D Ligands RAE-1δ and RAE-1ε Differ with Respect to Their Receptor Affinity, Expression Profiles and Transcriptional Regulation
    Article Snippet: Transfection efficiency was assessed by the co-tranfection of pRL-null plasmid (Promega) encoding Renilla luciferase.



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    renilla luciferase plasmid prl-null - by Bioz Stars, 2026-09
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    (A-B) IFN-β and NF-κB promoter activities are downregulated in DF-1 TLR3 KO cells but can be rescued by exogenous expression of TLR3. DF-1 and DF-1 TLR3 KO cells were co-transfected with pLucter (100 ng) and pR-null (30 ng) plasmids (A) , or with pSI-chNFκB-Luc (50 ng) plasmid (B) together with different amounts (100, 200, 400 or 800 ng) of the plasmid expressing chTLR3-His. At 8 h pt the cells were transfected with 250 ng of Poly I:C, and harvested at 24 h after plasmid transfection. A control consisting in cells co-transfected with pLucter, pR-null and the empty pCDNA3, or with the pSI-chNFκB-Luc, and the empty pCDNA3 plasmid (800 ng) was included in each assay. The samples were analyzed by the dual luciferase assay kit. Each determination was carried out in duplicate, and the firefly luciferase expression level of each sample was normalized by Renilla values. Results are expressed as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not transfected with poly I:C. (C-D) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) (C) or with the plasmid pSI-chNFκB-Luc (50 ng) (D). At 8 h pt, cells were either treated with Poly I:C (20 µg) added directly to the culture medium or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase activity quantification. (E) DF-1 and DF-1 TLR3 KO cells were transfected with a siRNA specifically silencing the expression of MDA5 protein, or with a control siRNA. At 24 h pt, the cultures were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) and 8 h later cells were either treated with poly I:C (20μg) or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase assay. (F) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng). At 8 h pt, cells were transfected with 250 ng of Poly I:C and subsequently treated with BFA (50μM) or the vehicle DMSO 1 h later. The samples were harvested at 24 h after plasmid transfection and analyzed using the dual luciferase assay kit. In panels C-F values are presented as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not treated with Poly I:C. Bars indicate means ± standard deviations based on data of duplicate samples from three independent experiments. *, ** and *** indicate p values of <0.05, <0.01 and <0.001, respectively, as determined by unpaired Student’s test. ns, not significant.

    Journal: bioRxiv

    Article Title: Key role of TLR3 in type I IFN expression and apoptosis induction in IBDV-infected chicken fibroblast cells

    doi: 10.1101/2025.07.16.665101

    Figure Lengend Snippet: (A-B) IFN-β and NF-κB promoter activities are downregulated in DF-1 TLR3 KO cells but can be rescued by exogenous expression of TLR3. DF-1 and DF-1 TLR3 KO cells were co-transfected with pLucter (100 ng) and pR-null (30 ng) plasmids (A) , or with pSI-chNFκB-Luc (50 ng) plasmid (B) together with different amounts (100, 200, 400 or 800 ng) of the plasmid expressing chTLR3-His. At 8 h pt the cells were transfected with 250 ng of Poly I:C, and harvested at 24 h after plasmid transfection. A control consisting in cells co-transfected with pLucter, pR-null and the empty pCDNA3, or with the pSI-chNFκB-Luc, and the empty pCDNA3 plasmid (800 ng) was included in each assay. The samples were analyzed by the dual luciferase assay kit. Each determination was carried out in duplicate, and the firefly luciferase expression level of each sample was normalized by Renilla values. Results are expressed as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not transfected with poly I:C. (C-D) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) (C) or with the plasmid pSI-chNFκB-Luc (50 ng) (D). At 8 h pt, cells were either treated with Poly I:C (20 µg) added directly to the culture medium or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase activity quantification. (E) DF-1 and DF-1 TLR3 KO cells were transfected with a siRNA specifically silencing the expression of MDA5 protein, or with a control siRNA. At 24 h pt, the cultures were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng) and 8 h later cells were either treated with poly I:C (20μg) or transfected with increasing amounts (100, 200 and 300 ng) of Poly I:C. The samples were harvested at 24 h after plasmid transfection and used for luciferase assay. (F) DF-1 and DF-1 TLR3 KO cells were transfected with the plasmids pLucter (100 ng) and pR-null (30 ng). At 8 h pt, cells were transfected with 250 ng of Poly I:C and subsequently treated with BFA (50μM) or the vehicle DMSO 1 h later. The samples were harvested at 24 h after plasmid transfection and analyzed using the dual luciferase assay kit. In panels C-F values are presented as the fold induction over the level found in the control DF-1 or DF-1 TLR3 KO cells not treated with Poly I:C. Bars indicate means ± standard deviations based on data of duplicate samples from three independent experiments. *, ** and *** indicate p values of <0.05, <0.01 and <0.001, respectively, as determined by unpaired Student’s test. ns, not significant.

    Article Snippet: The Renilla luciferase plasmid (pRL-null; Promega) was kindly provided for Dr. Pablo Gastaminza.

    Techniques: Expressing, Transfection, Plasmid Preparation, Control, Luciferase, Activity Assay

    A , B WT and 4E KO MEF ( A ), or shScr and sh4EBP1/2 HEK293 cells ( B ) were grown in complete medium or glucose starved (Glc strv) for the indicated times, and analyzed by immunoblotting using antibodies against the indicated proteins. Representative results of two independent experiments are shown. C ShScr and sh4EBP1/2 HEK293 cells were grown in complete medium or glucose (Glc) starved for 16 h, and ACACA mRNA expression was analyzed by qRT-PCR. D ShScr and sh4EBP1/2 HEK293 cells were grown in complete medium or glucose (Glc) starved for 6 h, and translation efficiency (TE) of ACACA mRNA was calculated by measuring the levels of polysomal and total ACACA mRNA by qRT-PCR. n = 4 independent experiments. E , F WT and 4E KO MEF ( E ), or shScr and sh4EBP1/2 HEK293 cells ( F ) were transfected with an ACACA 5’UTR-containing Firefly Luciferase construct and a control Renilla Luciferase vector. Cells were grown in complete medium or glucose (Glc) starved for 6 h, and luminescence was measured. G HEK293 cells were transfected with an HA-tagged ACC1 expressing vector containing or not the ACACA 5’UTR. Cells were grown in complete medium or glucose starved (Glc strv) for the indicated times, and analyzed by immunoblotting using antibodies against the indicated proteins. Representative results of three independent experiments are shown. H , I 4E KO MEF ( H ) or sh4EBP1/2 HEK293 cells ( I ) were transfected with control siRNA (scr) or siRNAs targeting ACACA and grown in glucose starved medium (Glc strv) for 48 h. Cell death was measured by PI staining and flow cytometry. ACC1 protein levels were analyzed by immunoblotting. Data are shown as the mean ± SD. Statistics: unpaired one-sided Student’s t test ( C – F , H , I ); n = 3 independent experiments for ( C , E , F , H , I ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: mTORC1 regulates cell survival under glucose starvation through 4EBP1/2-mediated translational reprogramming of fatty acid metabolism

    doi: 10.1038/s41467-024-48386-y

    Figure Lengend Snippet: A , B WT and 4E KO MEF ( A ), or shScr and sh4EBP1/2 HEK293 cells ( B ) were grown in complete medium or glucose starved (Glc strv) for the indicated times, and analyzed by immunoblotting using antibodies against the indicated proteins. Representative results of two independent experiments are shown. C ShScr and sh4EBP1/2 HEK293 cells were grown in complete medium or glucose (Glc) starved for 16 h, and ACACA mRNA expression was analyzed by qRT-PCR. D ShScr and sh4EBP1/2 HEK293 cells were grown in complete medium or glucose (Glc) starved for 6 h, and translation efficiency (TE) of ACACA mRNA was calculated by measuring the levels of polysomal and total ACACA mRNA by qRT-PCR. n = 4 independent experiments. E , F WT and 4E KO MEF ( E ), or shScr and sh4EBP1/2 HEK293 cells ( F ) were transfected with an ACACA 5’UTR-containing Firefly Luciferase construct and a control Renilla Luciferase vector. Cells were grown in complete medium or glucose (Glc) starved for 6 h, and luminescence was measured. G HEK293 cells were transfected with an HA-tagged ACC1 expressing vector containing or not the ACACA 5’UTR. Cells were grown in complete medium or glucose starved (Glc strv) for the indicated times, and analyzed by immunoblotting using antibodies against the indicated proteins. Representative results of three independent experiments are shown. H , I 4E KO MEF ( H ) or sh4EBP1/2 HEK293 cells ( I ) were transfected with control siRNA (scr) or siRNAs targeting ACACA and grown in glucose starved medium (Glc strv) for 48 h. Cell death was measured by PI staining and flow cytometry. ACC1 protein levels were analyzed by immunoblotting. Data are shown as the mean ± SD. Statistics: unpaired one-sided Student’s t test ( C – F , H , I ); n = 3 independent experiments for ( C , E , F , H , I ). Source data are provided as a Source Data file.

    Article Snippet: For transfection, HEK293 cells were seeded in 12-well plates and transfected with 250 ng of each 5’UTR Firefly Luciferase reporter and 3 ng Renilla Luciferase expressing pRL null plasmid (Promega), completed to 500 ng DNA with pcDNA3.1 plasmid, using CalFectin transfection reagent (Signagen) according to the manufacturer’s guidelines.

    Techniques: Western Blot, Expressing, Quantitative RT-PCR, Transfection, Luciferase, Construct, Plasmid Preparation, Staining, Flow Cytometry